Adrenal Gland and Kidney in Controlling Hypertension

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Adrenal Gland and Kidney in Controlling Hypertension Essay

Adrenal glands are micro-tissues located at the top of each kidney. Adrenal glands produce hormones that are essential to the functionality of the body. Among the hormones that adrenal glands produce include sex hormones and cortisol hormones that control several functions, including stress management (Huether & McCance 2015). The kidneys are two bean-shaped organs located in the lower abdomen of the body whose main factions is to clean the blood and remove toxic substances from the body. The kidney and the adrenal glands work together to perform several other functions. However, this essay focuses explicitly on the role of the two organs in controlling hypertension.

In nursing, it is not enough to conclude that the kidney and the adrenal glands control blood pressure without highlighting the roles that each of the organs plays distinctively in controlling the blood pressure. Adrenal Gland and Kidney in Controlling Hypertension Essay. Although it is common knowledge that Angiotensin II hormones acting through type 1 Angiotensin are the vital agents of hypertension control, adrenal glands and kidney are very instrumental in producing the chemical components that form these hormones (Crowley et al., 2005). The kidney controls sodium excretion, which forms a critical part of the mechanism of blood pressure control. The adrenal gland, on the other hand, produces the cortisol hormones which perform numerous functions including regulating blood pressure and increasing blood sugar levels. The communication between the adrenal glands and the pituitary glands of the brain triggers the formation of hormones based on the body conditions that require the formation of the hormones (Guyenet, 2006). For example, when blood pressure increases, the pituitary glands send nervous signals to the adrenal glands prompting the glands and the kidneys to produce hormones that control the situation.

References

Huether, S. E., & McCance, K. L. (2015). Understanding Pathophysiology-E-Book. Elsevier Health Sciences.

Crowley, S. D., Gurley, S. B., Oliverio, M. I., Pazmino, A. K., Griffiths, R., Flannery, P. J., … & Coffman, T. M. (2005). Distinct roles for the kidney and systemic tissues in blood pressure regulation by the renin-angiotensin system. The Journal of clinical investigation, 115(4), 1092-1099.

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Guyenet, P. G. (2006). The sympathetic control of blood pressure. Nature Reviews Neuroscience, 7(5), 335. Adrenal Gland and Kidney in Controlling Hypertension Essay.

Adrenal glands are micro-tissues located at the top of each kidney. Adrenal glands produce hormones that are essential to the functionality of the body. Among the hormones that adrenal glands produce include sex hormones and cortisol hormones that control several functions, including stress management (Huether & McCance 2015). The kidneys are two bean-shaped organs located in the lower abdomen of the body whose main factions is to clean the blood and remove toxic substances from the body. The kidney and the adrenal glands work together to perform several other functions. However, this essay focuses explicitly on the role of the two organs in controlling hypertension.

In nursing, it is not enough to conclude that the kidney and the adrenal glands control blood pressure without highlighting the roles that each of the organs plays distinctively in controlling the blood pressure. Although it is common knowledge that Angiotensin II hormones acting through type 1 Angiotensin are the vital agents of hypertension control, adrenal glands and kidney are very instrumental in producing the chemical components that form these hormones (Crowley et al., 2005). The kidney controls sodium excretion, which forms a critical part of the mechanism of blood pressure control. The adrenal gland, on the other hand, produces the cortisol hormones which perform numerous functions including regulating blood pressure and increasing blood sugar levels.Adrenal Gland and Kidney in Controlling Hypertension Essay. The communication between the adrenal glands and the pituitary glands of the brain triggers the formation of hormones based on the body conditions that require the formation of the hormones (Guyenet, 2006). For example, when blood pressure increases, the pituitary glands send nervous signals to the adrenal glands prompting the glands and the kidneys to produce hormones that control the situation.

References

Huether, S. E., & McCance, K. L. (2015). Understanding Pathophysiology-E-Book. Elsevier Health Sciences.

Crowley, S. D., Gurley, S. B., Oliverio, M. I., Pazmino, A. K., Griffiths, R., Flannery, P. J., … & Coffman, T. M. (2005). Distinct roles for the kidney and systemic tissues in blood pressure regulation by the renin-angiotensin system. The Journal of clinical investigation, 115(4), 1092-1099.

Guyenet, P. G. (2006). The sympathetic control of blood pressure. Nature Reviews Neuroscience, 7(5), 335.

Primary aldosteronism is the most common cause of secondary hypertension. In the past, screening for primary aldosteronism was offered only in patients with hypertension associated with hypokalemia. Recent studies showed that hypokalemia is seen in only 25% of the patients with primary aldosteronism, which has increased the prevalence of primary aldosteronism to 10–15% of all cases with new onset hypertension. Adrenal Gland and Kidney in Controlling Hypertension Essay.

  1. Introduction
    Approximately 85 million people in the United States are estimated to be hypertensive. In the majority, the hypertension is “essential” or “idiopathic,” but a subgroup of approximately 15% has secondary hypertension. The secondary causes of hypertension can be divided into renal (e.g., renovascular or renal parenchymal disease) and endocrine causes. There are at least 14 endocrine disorders in which hypertension may be the initial clinical presentation (Table 1).

Adrenal dependent
(1) Pheochromocytoma
(2) Cushing’s syndrome
(3) Primary hyperaldosteronism
(4) Other adrenocortical tumors (i.e., carcinoma, other)
(5) Genetic defects affecting adrenocortical function
    (i) Congenital adrenal hyperplasia: 11β-Hydroxylase and 17α-Hydroxylase deficiency, primarily
    (ii) Primary cortisol resistance
Apparent mineralocorticoid excess (AME)/11β-Hydroxysteroid dehydrogenase deficiency
  Genetic
   (i) Type 1 AME
  (ii) Type 2 AME
 Acquired
   (i) Licorice or carbenoxolone ingestion (type 1 AME)
    (ii) Cushing’s syndrome (type 2 AME)
 Thyroid dependent
 Hyperthyroidism
  Parathyroid dependent
  Hyperparathyroidism
  Pituitary dependent
   (1) Acromegaly
   (2) Cushing’s disease
Table 1
Endocrine causes of hypertension.
A further classification of the adrenocortical causes of hypertension based on the levels of renin and aldosterone can be used for the diagnostic approach of a patient with suspected endocrine hypertension (Table 2). Adrenal Gland and Kidney in Controlling Hypertension Essay.

      Low renin and high aldosterone
Primary aldosteronism
   (1) Aldosterone producing adenomas(APA)—35% of cases
   (2) Bilateral idiopathic hyperplasia(IHA)—60% of cases
   (3) Primary adrenal hyperplasia—2% of cases
   (4) Aldosterone-producing adrenocortical carcinoma—<1% of cases
   (5) Familial Hyperaldosteronism(FH)
       (i) Glucocorticoid-remediable Aldosteronism(FH type I)—<1% of cases
       (ii) FH type II(APA or IHA)—<2% of cases
  (6) Ectopic aldosterone producing adenoma or carcinoma-<0.1% of cases
     Low renin and low aldosterone
Hyperdeoxycorticosteronism
   (1) Congenital adrenal hyperplasia
     11β-hydroxylase deficiency
     17α-hydroxylase deficiency
   (2) Deoxycorticosterone producing tumor
   (3) Primary cortisol resistance
    (4) Apparent mineralocorticoid excess (AME) 11β-Hydroxysteroid dehydrogenase deficiency (Genetic or Acquired)
    (5) Cushing’s syndrome and Cushing’s disease
Table 2
Adrenocortical causes of hypertension.
An accurate diagnosis of endocrine hypertension provides the clinician with a unique treatment opportunity, that is, to render a surgical cure or to achieve a dramatic response with pharmacologic therapy. Primary hyperaldosteronism, which represents the most common cause of endocrine resistant hypertension, is reviewed here.

  1. Aldosterone Biosynthesis and Control of Secretion
    The differentiation of the adrenal cortex into distinct zones has important functional consequences. The zona glomerulosa comprises approximately 15% of the cortex (depending upon sodium intake). Cells are clustered in spherical nests and are small with smaller nuclei in comparison to other zones. The zona fasciculata comprises the 75% of the cortex; in this zone, the cells are large and lipid laden and form radial cords between the fibrovascular radial network. The innermost zona reticularis is sharply demarcated from both zona fasciculata and the adrenal medulla; cells are irregular with little lipid content. Three main types of hormone are produced by the adrenal cortex-glucocorticoids (cortisol, corticosterone), mineralocorticoids (aldosterone, deoxycorticosterone), and sex steroids (mainly androgens).

Aldosterone is synthesized from cholesterol in a series of six biosynthetic steps [1]. The first four steps are also involved in the synthesis of cortisol, whereas the last two pertain only to aldosterone [2]. The product of the CYP11B2 gene is capable of catalyzing both the 11-hydroxylase and 18-hydroxylase and 18-hydroxydehydrogenase steps in aldosterone biosynthesis [2–4]. The CYP11B2gene is located on human chromosome 8q24.3-tel [5].

Aldosterone, the major circulating mineralocorticoid, is a steroid hormone produced exclusively in the zona glomerulosa. The major regulators of aldosterone biosynthesis and secretion are the renin-angiotensin system and potassium ion concentrations. Minor regulators include corticotrophin (ACTH) from the pituitary, atrial natriuretic peptide from the heart, and dopamine secreted locally in the adrenal. Adrenal Gland and Kidney in Controlling Hypertension Essay.A number of aldosterone precursors, including deoxycorticosterone and 18-hydroxycorticosterone, have mineralocorticoid activity, and their hypersecretion in various pathological states may produce or exacerbate features typical of mineralocorticoid hypertension. Aldosterone acts mainly on the distal nephron although several other sites of sodium reabsorption exist.

The classic functions of aldosterone are regulation of extracellular volume and control of potassium homeostasis. These effects are mediated by binding of free aldosterone to the mineralocorticoid receptor in the cytosol of epithelial cells principally in the kidney. Mineralocorticoid receptors have a tissue-specific expression. For example, tissues with the highest concentration of these receptors are the distal nephron, hippocampus, and colon. Lower concentrations are found in the rest of the gastrointestinal tract and heart. Transport to the nucleus and binding to specific binding domains on targeted genes lead to their increased expression. Aldosterone-regulated kinase appears to be a key intermediary, and its increased expression leads to modification of the apical sodium channel, resulting in increased sodium ion transport across the cell membrane. The increased tubular negativity augments tubular secretion of potassium by the tubular cells and hydrogen ion by the interstitial cells.

Glucocorticoids and mineralocorticoids bind equally to the mineralocorticoid receptor. Specificity of action is provided by the presence of a glucocorticoid metabolizing enzyme, 11β-hydroxysteroid dehydrogenase (11BHSD2), which metabolizes cortisol to cortisone and prevents glucocorticoids from interacting with the receptor.

In addition to the classic genomic actions mediated by aldosterone binding to cytosolic receptors, mineralocorticoids have acute, nongenomic actions due to activation of an unidentified cell surface receptor. This action involves most likely a G protein-coupled receptor and probably a modification of the sodium hydrogen exchange activity.

Aldosterone has additional, nonclassic effects primarily on non epithelial cells. These include the expression of several collagen genes, genes controlling tissue growth factors, such as transforming growth factor β and plasminogen activator inhibitor type 1, and genes mediating inflammation. The resultant actions lead to microangiopathy, necrosis (acutely), and fibrosis in various tissues such as the heart, the vasculature, and the kidney.

At the level of the zona glomerulosa, the major stimulatory influences are angiotensin II and serum potassium [6, 7]. ACTH stimulates aldosterone secretion in an acute but transient fashion, but it is questionable whether ACTH plays a significant role in the chronic regulation of mineralocorticoid secretion [8]. The major inhibitory influences affecting the zona glomerulosa are exerted by circulating atrial natriuretic peptide (ANP) and locally by dopamine [9].Adrenal Gland and Kidney in Controlling Hypertension Essay. Although ANP levels are clearly increased in hyperaldosteronism, neither ANP nor dopamine has been implicated as primary causes of clinically significant defects in aldosterone secretion. Metoclopramide increases aldosterone secretion, suggesting dopamine may inhibit aldosterone release [10–12]. The physiologic roles of adrenomedullin and vasoactive intestinal peptide (VIP) on aldosterone secretion remain to be clarified; it appears that both these neuropeptides are produced in the rat zona glomerulosa [13, 14].

  1. Primary Hyperaldosteronism
    In his presidential address at the Annual Meeting of the Central Society for Clinical research, Chicago Illinois, October 29 1954, Dr. Jerome W. Conn stated: “I have prepared no comprehensive review of my personal philosophy of clinical investigation. Instead, I plan to make a scientific report to you about a clinical syndrome, the investigation of which has been most exciting to me since I initiated it in April of this year”.

In April 1954, Conn was asked to see M. W., a 34-year-old woman with a 7-year history of muscle spasms, temporary paralysis, tetany, and weakness and a 4-year history of hypertension. Because there were no signs or symptoms of glucocorticoid or androgen excess, Conn suspected based on his prior research that M. W.’s clinical presentation could result from excess secretion of the adrenal salt-retaining corticoid. Conn planned for a bilateral adrenalectomy on December 1954. In 1955, Gittler and Fajans told of the surgical scene: To “the immense delight of Conn and those in the operating room, the surgeon, Dr. William Baum, encountered a right 13gr adrenal tumor which was removed while leaving the contralateral gland intact. The patient’s post operative studies showed an almost total reversal of the preoperative metabolic and clinical abnormalities”.

By 1964, Conn had collected 145 cases, and he suggested that up to 20% of patients with essential hypertension might have primary aldosteronism. Later, Conn decreased his predicted prevalence or primary aldosteronism to 10% of hypertensives, a prediction that was substantiated nearly 40 years later.

In the past, clinicians would not consider the diagnosis of primary hyperaldosteronism unless the patient presented with spontaneous hypokalemia, and then the diagnostic evaluation would require discontinuation of antihypertensive medications for at least 2 weeks. The spontaneous hypokalemia/no antihypertensive drug approach resulted in predicted prevalence rates of less than 0.5% of hypertensive patients. Adrenal Gland and Kidney in Controlling Hypertension Essay.

However, it is now recognized that most patients with primary aldosteronism are not hypokalemic and that screening can be completed with a simple blood test (plasma aldosterone concentration [PAC]-to-plasma renin activity (PRA) ratio) while the patient is taking antihypertensive drugs. Using the PAC/PRA ratio as a screening test, followed by confirmatory testing, has resulted in much higher prevalence estimates (5% to 13% of all patients with hypertension) for primary aldosteronism.

Since Conn described the first case of an aldosterone-producing adenoma, several subtypes of primary aldosteronism have been described (Table 2). The differential diagnosis of these subtypes is crucial for the management and the prognosis.

  1. Clinical Presentation
    The diagnosis of primary aldosteronism is usually made in patients who are in the third to sixth decade of life. In cases associated with hypokalemia, patients may have muscle spasms, weakness, headaches, palpitations, polyuria, polydipsia, nocturia, or a combination of these. Periodic paralysis is a very rare presentation in Caucasians but not an infrequent presentation in patients of Asian descent. Sometimes, tetany can develop due to decreased ionized calcium from hypokalemic alkalosis. The nocturia and polyuria are a result of hypokalemia-induced renal concentrating defect, and the presentation is frequently mistaken for prostatic disease in men.

There are no specific physical findings. Edema is not a common finding because of the mineralocorticoid escape phenomenon. The degree of hypertension may be moderate to severe and may be resistant to usual pharmacologic treatments. Patient with aldosterone producing adenoma tend to have higher blood pressure compared to patient with bilateral idiopathic hyperplasia. Hypokalemia is frequently absent; in some patients, the hypokalemia can become evident only with the addition of potassium-wasting diuretics, and some patients can develop profound hypokalemia with only small doses of diuretics. Because of a reset osmostat, the serum sodium concentration tends to be high normal or above the upper limit of normal. This clinical clue is very useful when initially assessing the potential for primary aldosteronism. Adrenal Gland and Kidney in Controlling Hypertension Essay.

Patients with primary aldosteronism may be at higher risk than other patients with essential or secondary hypertension for target-organ damage of the heart and kidney. That can be explained from a direct effect of aldosterone on these organs.

  1. Screening for Primary Aldosteronism
    The diagnostic approach to primary aldosteronism can be considered in three phases: screening, confirmation, followed by subtype evaluation tests.

Physicians should always consider screening for primary aldosteronism in the following situations:(1)hypertension and hypokalemia,(2)resistant hypertension,(3)adrenal incidentaloma and hypertension,(4)onset of hypertension at a young age (<20 years),(5)severe hypertension (>160 mm systolic or >100 diastolic),(6)secondary hypertension,(7)profound hypokalemia with usual doses of potassium wasting diuretics.

The initial screening tests include the measurement of plasma aldosterone concentration and plasma renin activity and calculation of the PAC/PRA (ARR) ratio. Like all biochemical case detection tests, the ARR is not without false positives and negatives. Table 2 documents the effect of medications and conditions on the ARR. The ARR should, therefore, be regarded as a detection test only and should be repeated if the initial results are inconclusive or difficult to interpret because of suboptimal sampling conditions.

If a patient is already on any of the medications listed in Table 3, he can be switched over to medications [15, 16] that do not affect or affect minimally the ARR. In Table 4 are listed the antihypertensive medications that have minimal effects on plasma aldosterone levels and can be used to control hypertension during case finding and confirmatory testing for primary aldosteronism [15, 16].

PAC PRA PAC/PRA
Medications
 β-Adrenergic blockers ↓ ↓↓ ↑(FP)
 Central α-2 agonists ↓ ↓↓ ↑(FP)
 NSAIDs ↓ ↓↓ ↑(FP)
 K+-wasting diuretics →↑ ↑↑ ↓(FN)
 K+-sparing diuretics ↑ ↑↑ ↓(FN)
 ACE inhibitors ↓ ↑↑ ↓(FN)
 ARBs ↓ ↑↑ ↓(FN)
 Ca2+ blockers (DHPs) →↓ ↑ ↓(FN)
 Renin inhibitors ↓ ↓↑1 ↑(FP)1
↓ (FN) 1
Potassium status
 Hypokalemia ↓ →↑ ↓(FN)
 Potassium loading ↑ →↓ ↑(FP)
Dietary sodium
 Sodium restricted ↑ ↑↑ ↓(FN)
 Sodium loaded ↓ ↓↓ ↑(FP)
Advancing age ↓ ↓↓ ↑ (FP)
Other conditions
 Renal impairment → ↓ ↑(FP)
 PHA-2 → ↓ ↑(FP)
 Pregnancy ↑ ↑↑ ↓(FN)
 Renovascular HTN ↑ ↑↑ ↓(FN)
 Malignant HTN ↑ ↑↑ ↓ (FN)
Table 3
Drug Class Usual dose Comments
Verapamil slow release Nondihydropyridine calcium channel antagonist 90–120 mg twice daily Use singly or in combination with the other agents listed in this table.
Hydralazine Vasodilator 10–12.5 mg twice daily, increasing as required Commence verapamil slow release first to prevent reflex tachycardia. Commencement at low doses reduces risk of side effects (including headaches, flushing, and palpitations).
Prazosin hydrochloride α-Adrenergic blocker 0.5–1 mg two to three times daily, increasing as required Monitor for postural hypotension
Doxazosin mesylate α-Adrenergic blocker 1–2 mg once daily, increasing as required Monitor for postural hypotension
Terazosin hydrochloride α-Adrenergic blocker 1–2 mg once daily, increasing as required Monitor for postural hypotension
Table 4
The ARR is most sensitive when used in patients from whom samples are collected in the morning after patients have been out of bed for at least 2 h, usually after they have been seated for 5–15 min and without posture stimulation. Ideally, patients should have unrestricted dietary salt intake before testing. In many cases, the ARR can be confidently interpreted with knowledge of the effect on the ARR of continued medications or suboptimal conditions of testing, avoiding delay and allowing the patient to proceed directly to confirmatory/exclusion testing [15, 16]. Washout of all interfering antihypertensive medications is feasible in patients with mild hypertension but is potentially problematic in others and perhaps unnecessary in that medications with minimal effect on the ARR can be used in their place (Table 4). Adrenal Gland and Kidney in Controlling Hypertension Essay.

A suggested approach [15, 16] for measurement of ARR as per the 2008 Endocrine Society guidelines follow.

(A) Preparation for ARR Measurement: Agenda
(1)Attempt to correct hypokalemia, after measuring plasma potassium in blood collected slowly with a syringe and needle (preferably not a Vacutainer to minimize the risk of spuriously raising potassium); avoid fist clenching during collection; wait at least 5 sec after tourniquet release (if used to achieve insertion of needle); ensure separation of plasma from cells within 30 min of collection.(2)Encourage patient to liberalize (rather than restrict) sodium intake.(3)Withdraw agents that markedly affect the ARR (48) for at least 4 weeks:(a)spironolactone, eplerenone, amiloride, and triamtereneb,(b)potassium-wasting diureticsc,(c)products derived from licorice root (e.g., confectionary licorice, chewing tobacco).(4)If the results of ARR of the above agents are not diagnostic, and if hypertension can be controlled with relatively noninterfering medications (see Table 4), withdraw other medications that may affect the ARR (48) for at least 2 weeks like(a)β-Adrenergic blockers, central α-2 agonists (e.g., clonidine andα-methyldopa), nonsteroidal anti-inflammatory drugs,(b)angiotensin-converting enzyme inhibitors, angiotensin receptor blockers, renin inhibitors, and dihydropyridine calcium channel antagonists.(5)If necessary to maintain hypertension control, commence other antihypertensive medications that have lesser effects on the ARR (e.g., verapamil slow release, hydralazine (with verapamil slow release, to avoid reflex tachycardia), prazosin, doxazosin, and terazosin; see Table 4).(6)Establish OC and HRT status, because estrogen-containing medications may lower DRC and cause false-positive ARR when DRC (rather than PRA) is measured. Do not withdraw OC unless confident of alternative effective contraception.

(B) Conditions for Collection of Blood
(1)Collect blood mid-morning, after the patient has been up (sitting, standing, or walking) for at least 2 h and seated for 5–15 min.(2)Collect blood carefully, avoiding stasis and hemolysis (see A.1 above).(3)Maintain sample at room temperature (and not on ice, because this will promote conversion of inactive to active renin) during delivery to laboratory and before centrifugation and rapid freezing of plasma component pending assay.

(C) Factors to Take into Account when Interpreting Results (see Table 3)
(1)Age: in patients aged >65 yr, renin can be lowered more than aldosterone by age alone, leading to a raised ARR.(2)Time of day, recent diet, posture, and length of time in that posture.(3)Medications.(4)Method of blood collection, including any difficulty doing so.(5)Level of potassium. Level of creatinine (renal failure can lead to false-positive ARR). Adrenal Gland and Kidney in Controlling Hypertension Essay.
Another important consideration when we are screening patients for primary aldosteronism is the reliability and the sensitivity of the assay we use for both aldosterone and plasma renin activity. Because the ARR is mathematically highly dependent on renin, renin assays should be sufficiently sensitive to measure levels as low as 0.2–0.3 ng/mL·h (DRC 2 mU/liter). Although most laboratories use radioimmunoassay (RIA) for plasma and urinary aldosterone, measured levels of standards have been shown to be unacceptably different in some instances. Tandem mass spectrometry is increasingly used and has proved to be much more consistent in performance.

  1. Interpretation of ARR
    Secondary hyperaldosteronism should be considered when both PRA and PAC are increased and the ARR is less than 10 (e.g., Renovascular disease). If both PAC and PRA are suppressed, an alternate source of mineralocorticoid receptor agonist should be considered (e.g., hypercortisolism). Primary aldosteronism should be suspected when PRA is suppressed (<1 ng/mL/hr) and PAC is increased. It is important to understand that the lower limit of detection varies among different PRA assays and can have dramatic effect on the PAC/PRA ratio. For example, if the lower limit for detection of for PRA is 0.6 ng/mL/hr and the PAC is 16 ng/dL, then the ARR would be 27. However, if the lower limit for detection of PRA is 0.1 ng/mL/hr, then the ARR would be 160. Thus, the ARR cutoff for a high ARR is laboratory dependent and more specifically PRA assay dependent.

In a retrospective study [17], the combination of an ARR>30 and PAC >20 ng/dL had a sensitivity of 90% and a specificity of 91% for aldosterone producing adenomas. At Mayo clinic, an ARR of more than 20 and a PAC >15 ng/dL are found in more than 90% of patients with surgically confirmed aldosterone producing adenomas.

Most groups, however, use cutoffs of 20–40 when testing is performed in the morning on a seated ambulatory patient. Some investigators [18] require elevated aldosterone levels in addition to elevated ARR for a positive screening test for PA (usually aldosterone >15 ng/dL.) An alternative approach is to avoid a formal cutoff level for plasma aldosterone but to recognize that the likelihood of a false-positive ARR becomes greater when renin levels are very low. Adrenal Gland and Kidney in Controlling Hypertension Essay.

Against a formal cutoff level for aldosterone are the findings of several studies. In one study, seated plasma aldosterone levels were less than 15 ng/d in 36% of 74 patients diagnosed with PA after screening positive by ARR defined as more than 30 and showing failure of aldosterone to suppress during fludrocortisone suppression testing (FST) and in four of 21 patients found by AVS to have unilateral, surgically correctable PA. Another study reported plasma aldosterone levels of 9–16 ng/dL in 16 of 37 patients diagnosed with PA by FST. Although it would clearly be desirable to provide firm recommendations for ARR and plasma aldosterone cutoffs, the variability of assays between laboratories and the divided literature to date makes it more prudent to point out relative advantages and disadvantages, leaving clinicians the flexibility to judge for themselves.

  1. Confirmatory Tests
    An increased ARR is not diagnostic by itself and confirmatory tests are required to demonstrate inappropriate aldosterone secretion. Treatment with spironolactone or eplerenone should not be started before the confirmation of primary aldosteronism. In patients already receiving treatment with spironolactone or eplerenone, the treatment should be held for at least 4–6 weeks before further diagnostic testing. Aldosterone suppression testing can be performed with orally administered sodium chloride and measurement of urinary aldosterone or with intravenous sodium chloride loading and measurement of PAC.
  2. Oral Sodium Loading Test
    After hypertension and hypokalemia are controlled, patients should increase their sodium intake to >200 mmol/d (~6 g/d) for 3 d, verified by 24-h urine sodium content. Patients should receive adequate slow-release potassium chloride supplementation to maintain plasma potassium in the normal range. Urinary aldosterone is measured in the 24-h urine collection from the morning of d 3 to the morning of d 4. Primary aldosteronism is unlikely if urinary aldosterone is lower than 10 μg/24 h (27.7 nmol/d) in the absence of renal disease, where primary aldosteronism may coexist with lower measured urinary aldosterone levels. Elevated urinary aldosterone excretion (>12 μg/24 h (>33.3 nmol/d) at the Mayo Clinic, >14 μg/24 h (38.8 nmol/d) at the Cleveland Clinic) makes primary aldosteronism highly likely. This test should not be performed in patients with severe uncontrolled hypertension, renal insufficiency, cardiac insufficiency, cardiac arrhythmia, or severe hypokalemia. The 24-h urine collection may be inconvenient. Adrenal Gland and Kidney in Controlling Hypertension Essay. Laboratory-specific poor performance of the RIA for urinary aldosterone (aldosterone 18-oxo-glucuronide or acid-labile metabolite) may blunt diagnostic accuracy, a problem obviated by the currently available HPLC-tandem mass spectrometry methodology. Aldosterone 18-oxo-glucuronide is a renal metabolite, and its excretion may not rise in patients with renal disease.
  3. Intravenous Saline Infusion Test
    The test is done after an overnight fast. Patients stay in the recumbent position for at least 1 h before and during the infusion of 2 liters of 0.9% saline iv over 4 h, starting at 0800–0930 h. Blood samples for renin, aldosterone, cortisol, and plasma potassium are drawn at time zero and after 4 h, with blood pressure and heart rate monitored throughout the test. Postinfusion plasma aldosterone levels <5 ng/dL make the diagnosis of primary aldosterone unlikely, and levels >10 ng/dL are a very probable sign of primary aldosterone. Values between 5 and 10 ng/dL are indeterminate and can be seen in patients with bilateral idiopathic hyperplasia. This test should not be performed in patients with severe uncontrolled hypertension, renal insufficiency, cardiac insufficiency, cardiac arrhythmia, or severe hypokalemia.
  4. Subtype Studies
    All patients with primary aldosteronism (PA) should undergo an adrenal CT scan as the initial study in subtype testing and to exclude large masses that may represent adrenocortical carcinoma. The findings on adrenal CT—normal-appearing adrenals, unilateral macroadenoma (>1 cm), minimal unilateral adrenal limb thickening, unilateral microadenomas (≤1 cm), or bilateral macro- or microadenomas (or a combination of the two)—are used in conjunction with adrenal venous sampling (AVS) and, if needed, ancillary tests to guide treatment decisions in patients with PA. Aldosterone-producing adenomas (APA) may be visualized as small hypodense nodules (usually <2 cm in diameter) on CT. Adrenal glands with bilateral idiopathic hyperplasia (IHA) may be normal on CT or show nodular changes. Aldosterone-producing adrenal carcinomas are almost always more than 4 cm in diameter, but occasionally smaller, and like most adrenocortical carcinomas have a suspicious imaging phenotype on CT.

Adrenal CT has several limitations. Small APAs may be interpreted incorrectly by the radiologist as IHA on the basis of CT findings of bilateral nodularity or normal-appearing adrenals. Moreover, apparent adrenal microadenomas may actually represent areas of hyperplasia, and unilateral adrenalectomy would be inappropriate. In addition, nonfunctioning unilateral adrenal macroadenomas are not uncommon, especially in older patients (>40 yr) and are indistinguishable from APAs on CT. Unilateral UAH may be visible on CT, or the UAH adrenal may appear normal on CT. Magnetic resonance imaging has no advantage over CT in subtype evaluation of PA, being more expensive and having less resolution than CT.

  1. Adrenal Venous Sampling
    Lateralization of the source of the excessive aldosterone secretion is critical to guide the management of PA. Distinguishing between unilateral and bilateral disease is important because unilateral adrenalectomy in patients with APA or UAH results in normalization of hypokalemia in all; hypertension is improved in all and cured in 30%–60% [19, 20]. In bilateral IHA and GRA, unilateral or bilateral adrenalectomy seldom corrects the hypertension, and medical therapy is the treatment of choice. Unilateral disease may be treated medically if the patient declines or is not a candidate for surgery. Adrenal Gland and Kidney in Controlling Hypertension Essay. ORDER A PLAGIARISM -FREE PAPER NOW

Adrenal vein sampling was initially proposed as a way to localize catecholamine secreting neoplasms (Young 2009). In 1967, Melby et al. reported the use of adrenal vein sampling in primary aldosteronism, and it has since become the gold standard test to differentiate unilateral from bilateral disease (Young 2009). In his initial paper, he reported the comparison of aldosterone from the right and left adrenal veins in seven patients with primary aldosteronism and also in three normal patients. The authors found elevated aldosterone secretion and a higher aldosterone to cortisol ratio from the involved adrenal gland compared to the uninvolved adrenal gland. All primary aldosteronism patients in the study were operated for unilateral adrenalectomy and were found to have adenomas that correlated with the lateralization found on adrenal vein sampling (Melby 1967).

From this first report, adrenal vein sampling in primary aldosteronism has continued to evolve. Some controversies remain including whether the addition of cosyntropin to the test either as a bolus or constant infusion improves the diagnostic accuracy and what diagnostic criteria to use (Auchus 2010). In performing this test, there are some things that need to be considered. First, the patient needs to be prepared properly. They should be tested in a fasting state and be supine for at least one hour before testing (Young 2009). Additionally, certain antihypertensives should be avoided to prevent false results. Mineralocorticoid receptor antagonists in particular should be discontinued at least 6 weeks before testing. Preferred medications include extended release verapamil, α-adrenergic blockers, and hydralazine. Patients should also be potassium repleted as hypokalemia can lower the aldosterone levels (Rossi 2007).

In addition to patient preparation, the test should be performed by an experienced and skilled practitioner, especially given some of the technical difficulties that can arise in cannulating the right adrenal vein. Additionally, a well-defined protocol should be in place (Rossi 2007, Young 2009). Samples are obtained from the right adrenal, left adrenal, and from a peripheral source. This should be performed simultaneously or in rapid sequence to limit fluctuations in secretion that occur with time (Young 2009).

The NIH protocol for adrenal vein sampling in primary aldosteronism is as follows. Adrenal Gland and Kidney in Controlling Hypertension Essay.